Curated reading path
A continuous path from ultra-large libraries, noncanonical amino acids and cyclization to selection trajectories and AI-assisted design.
5 published articles in recommended order
mRNA display uses a puromycin-mediated covalent linkage so that each peptide carries its own sequence identity, enabling iterative selection from libraries on the order of 10¹²–10¹³ molecules and supporting cyclic-peptide, NCAA and data-driven discovery.
mRNA display combines genotype–phenotype linkage, cell-free translation, genetic code reprogramming, noncanonical amino-acid incorporation, macrocyclization, and iterative selection in one traceable discovery system.
One round of mRNA display is a population process shaped by library design, translation, fusion formation, target selection, counter-selection, washing, recovery, amplification, and NGS.
The most useful AI signal from mRNA display is not a final-round hit list, but a selection trajectory combining enrichment, counter-selection, sequence families, NCAA and cyclization chemistry, and experimental validation.
PCSK9 was a validated yet difficult protein–protein interaction target. mRNA display found two cyclic-peptide hit series in a vast chemical space, but a binder was only the beginning of the drug-discovery journey.